Location and role of ion channels of the cochlea

Anthony Ricci, PhD, associate professor of otolaryngology, and colleagues at the University of Wisconsin and the Pellegrin Hospital in France found that the ion channels responsible for hearing aren't located where scientists previously thought. The discovery turns old theories upside down, and it could have major implications for the prevention and treatment of hearing loss

Location is important, because our entire theory of how sound activates these channels depends on it. Now we have to re-evaluate the model that we've been showing in textbooks for the last 30 years."

Deep inside the ear, specialized cells called "hair cells" sense vibrations in the air. The cells contain tiny clumps of hairlike projections, known as stereocilia, which are arranged in rows by height. Sound vibrations cause the stereocilia to bend slightly, and scientists think the movement opens small pores, called ion channels. As positively charged ions rush into the hair cell, mechanical vibrations are converted into an electrochemical signal that the brain interprets as sound.

But after years of searching, scientists still haven't identified the ion channels responsible for this process. To pinpoint the channels' location, Ricci and colleagues squirted rat stereocilia with a tiny water jet. As pressure from the water bent the stereocilia, calcium flooded into the hair cells. The researchers used ultrafast, high-resolution imaging to record exactly where calcium first entered the cells. Each point of entry marked an ion channel.

The results were surprising: Instead of being on the tallest rows of stereocilia, like scientists previously thought, Ricci's team found ion channels only on the middle and shortest rows.

Ion channels on hair cells not only convert mechanical vibrations into signals for the brain, but they also help protect the ear against sounds that are too loud. Through a process called adaptation, the ear adjusts the sensitivity of its ion channels to match the noise level in the environment. Most people are already familiar with this phenomenon, Ricci said, though they might not realize it. "If you watch TV in bed and you have the sound turned down low, you can hear fine when you're going to sleep," he said. "But then when you get up in the morning and turn on the news, you have to turn the volume up."

That's because at night, when everything is quiet, the ear turns up its amplifier to hear softer sounds. "But when you get up in the morning," Ricci said, "and the kids are running around and the dog is barking, the ear has to reset its sensitivity so you can hear in noisier conditions without hurting your ear."

Defects in the ear's adaptation process put people at risk for both age-related and noise-related hearing loss. Understanding adaptation is a fundamental step in preventing hearing loss, said Robert Jackler, MD, the Edward C. and Amy H. Sewall Professor in Otorhinolaryngology at Stanford.

"Many forms of hearing loss and deafness are due to disturbances in the molecular biology of the hair cell," said Jackler, who was not involved in the study. "When you understand the nuts and bolts of how the hair cell works, you can understand how it goes wrong and can set about learning how to fix it."

Other scientists have attempted similar experiments in the past, but they used less-sensitive imaging techniques. "Our microscope took images at 500 frames per second," said Ricci, who led the imaging experiments. "That's much faster than it's ever been done before."

Ricci and colleagues also used hair cells from rats, while previous experiments had been done in bullfrogs. Because mammals have fewer, more widely spaced rows of stereocilia, the team was able to determine the precise location of the ion channels.

Citations:

1. Stanford University Medical Center. "How Human Ear Translates Vibrations Into Sounds: Discovery Of Ion Channel Turns Ear On Its Head." ScienceDaily, 28 Apr. 2009. Web. 3 Mar. 2013.
2. Maryline Beurg, Robert Fettiplace, Jong-Hoon Nam & Anthony J Ricci. Localization of inner hair cell mechanotransducer channels using high-speed calcium imaging. Nature Neuroscience, 2009; DOI:10.1038/nn.2295